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Wenzhen LI, Yu DING, Qian SUI, Shuguang LU, Zhaofu QIU, Kuangfei LIN
《环境科学与工程前沿(英文)》 2012年 第6卷 第4期 页码 445-454 doi: 10.1007/s11783-012-0419-6
关键词:
clofibric acid
advanced oxidation processes
intermediates
toxicity
spp., foodborne pathogens threatening neonates and infants
Qiming CHEN, Yang ZHU, Zhen QIN, Yongjun QIU, Liming ZHAO
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 330-339 doi: 10.15302/J-FASE-2018208
Cronobacter spp. (formerly Enterobacter sakazakii) are special foodborne pathogens. Cronobacter infection can cause necrotizing enterocolitis, sepsis and meningitis in all age groups, especially neonates and infants, with a high fatality of up to 80%, although the infection is rare. Outbreaks of Cronobacter infection are epidemiologically proven to be associated with contaminated powdered infant formula (PIF). Cronobacter spp. can resist dry environments and survive for a long period in food with low water activity. Therefore, Cronobacter spp. have become serious pathogens of neonates and infants, as well as in the dairy industry. In this review, we present the taxonomy, pathogenesis, resistance, detection and control of Cronobacter spp.
关键词: Cronobacter spp. desiccation resistance pathogen control pathogen detection powdered infant formula
A CASE FOR ASSESSING ALLOCASUARINA AND CASUARINA SPP.
《农业科学与工程前沿(英文)》 2021年 第8卷 第4期 页码 568-582 doi: 10.15302/J-FASE -2019270
Agroecosystems in water-limited contexts— Mediterranean, semi-arid and arid climatic zones—are too frequently degraded systems that will not provide the needed ecosystem services to ensure a future of sustainable agricultural production. The processes that have created this situation continue and are being accelerated by anthropogenic climate change. Increasing arboreal vegetation in these areas through agroforestry is an important strategy to conserve and improve their agroecosystems. Actinorhizal trees and shrubs in the Casurinaceae have a unique set of adaptations for heat and water stress, and/or infertile to hostile soils. Central Anatolia, Turkey is particularly at risk of increasing aridity and further degradation. Therefore, species of Allocasuarina and Casuarina have been evaluated for their potential use in agroecosystem improvement in semi-arid areas with a focus on Central Anatolia. Based on a semiquantitative environmental tolerance index and reported plant stature, eight species were identified as being of high (A. verticillata and C. pauper) to moderate (A. acutivalvis, A. decaisneana, A. dielsiana, A. huegeliana, C. cristata and C. obesa) priority for assessment, with none of these species having been adequately evaluated for agroforestry deployment in semi-arid agroecosystems in any context.
关键词: actinorrhizal trees / agroforestry / climate change / ecosystem restoration
Repeats in the transcribed regions: comprehensive characterization and comparison of
Manosh Kumar BISWAS, Christoph MAYER, Xiuxin DENG
《农业科学与工程前沿(英文)》 2017年 第4卷 第4期 页码 421-432 doi: 10.15302/J-FASE-2017160
关键词: Citrus spp. tandem repeats transcribed region transposable elements
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
null
《医学前沿(英文)》 2017年 第11卷 第4期 页码 554-562 doi: 10.1007/s11684-017-0543-6
T cells engineered with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cell malignancy. However, many challenges remain in extending its application toward the treatment of solid tumors. The immunosuppressive nature of tumor microenvironment is considered one of the key factors limiting CAR-T efficacy. One negative regulator of T cell activity is lymphocyte activation gene-3 (LAG-3). We successfully generated LAG-3 knockout T and CAR-T cells with high efficiency using CRISPR-Cas9 mediated gene editing and found that the viability and immune phenotype were not dramatically changed during in vitro culture. LAG-3 knockout CAR-T cells displayed robust antigen-specific antitumor activity in cell culture and in murine xenograft model, which is comparable to standard CAR-T cells. Our study demonstrates an efficient approach to silence immune checkpoint in CAR-T cells via gene editing.
关键词: CAR-T CRISPR-Cas9 LAG-3
Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells
LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan
《医学前沿(英文)》 2007年 第1卷 第3期 页码 269-273 doi: 10.1007/s11684-007-0051-1
关键词: 3T3-L1 influence resistance receptor substrate-1 transport
Novel mutation c.1210-3C>G in with a poly-T tract of 5T affects mRNA splicing in a Chinese patient
《医学前沿(英文)》 2022年 第16卷 第1期 页码 150-155 doi: 10.1007/s11684-021-0846-5
Changes of phenotype and function of human CD4 CD25 T cells induced by transfection of Foxp3
WU Kui, BI Yutian, WANG Yaoli, WANG Changzheng
《医学前沿(英文)》 2008年 第2卷 第4期 页码 366-369 doi: 10.1007/s11684-008-0070-6
PAN Jiangang, ZHOU Xing, CHEN Zhiguang, HAN Ruifa
《医学前沿(英文)》 2008年 第2卷 第3期 页码 259-263 doi: 10.1007/s11684-008-0049-3
作为免疫疗法靶点的FOXP3及其辅因子 Review
Yasuhiro Nagai,Lian Lam,Mark I. Greene,Hongtao Zhang
《工程(英文)》 2019年 第5卷 第1期 页码 115-121 doi: 10.1016/j.eng.2019.01.001
叉头框蛋白P3(FOXP3)是调节性T细胞(Tregs)的一个主要调节因子,调节性T细胞是能抑制抗原特异性免疫反应的T 细胞亚群,在增强宿主耐受性和维持免疫平衡方面发挥着重要作用。众所周知,FOXP3 与多种蛋白质形成复合物,并能通过乙酰化、磷酸化、泛素化和甲基化等各种翻译后修饰(PTM)进行调节。因此,翻译后修饰可改变FOXP3 的稳定性及其调节基因表达的能力,并最终影响调节性T细胞活性。虽然FOXP3 自身并非理想的药物靶点,但脱乙酰酶、乙酰转移酶、激酶和其他可调节FOXP3 的翻译后修饰的酶均为调控FOXP3 和调节性T细胞活性的潜在靶点。但FOXP3 并非这些酶的唯一底物;因此,当使用相关抑制剂时,必须考虑是否存在有害的“FOXP3脱靶”副作用。
关键词: 调节性T细胞 叉头框蛋白P3(FOXP3) 翻译后修饰 自体免疫 癌症
Ductile extension of 3-D external circumferential cracks in pipe structures
Wuchao YANG, Xudong QIAN
《结构与土木工程前沿(英文)》 2011年 第5卷 第3期 页码 294-303 doi: 10.1007/s11709-011-0115-9
关键词:
ductile fracture
computational cell method
G-T model
募集抗原特异性T细胞的3D打印支架促进血管化 Article
李翠笛, 马振江, 李文韬, 颉天阳, 钟丽萍, 陈宏方, 王文豪, 王金武, 崔文国, 赵永祥
《工程(英文)》 2022年 第17卷 第10期 页码 183-195 doi: 10.1016/j.eng.2021.05.018
3D打印支架植入后激起的免疫反应是组织修复效果的主要决定性因素。因此,巧妙调控免疫反应的支架材料具有高效修复组织的潜能。本研究受抗原特异性免疫反应对血管新生促进作用的启发,通过动态共价键将介孔二氧化硅微棒(MSR)/聚乙烯亚胺(PEI)/卵清蛋白(OVA)自组装疫苗与3D打印自固化磷酸钙骨水泥(CPC)基支架结合,构建了一种具有调控局部体液免疫反应功能的组织修复支架通过局部释放OVA激活体液免疫反应,支架可有效募集抗原特异性CD4+ 2 型辅助性T细胞(Th2 细胞),从而促进支架植入早期的血管新生;在提供充足和丰富的血供环境的同时,MSR微棒中释放的硅离子还能有效加速骨再生在皮下包埋实验中,疫苗负载支架增加了Th2 细胞在脾脏中的比例,局部募集了抗原特异性T细胞并促进了支架内的血管新生。此外,原位颅骨缺损修复模型结果表明,负载疫苗的支架可促进血管化骨组织再生。
An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis
Dang-TRUNG,T. NGUYEN-THOI
《结构与土木工程前沿(英文)》 2015年 第9卷 第4期 页码 341-358 doi: 10.1007/s11709-015-0302-1
关键词: cracked Reissner-Mindlin shell free vibration analysis cell-based smoothed discrete shear gap method (CS-FEM-DSG3) extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) smoothed finite element methods (SFEM)
Yun Zhou, Siqing Xia, Binh T. Nguyen, Min Long, Jiao Zhang, Zhiqiang Zhang
《环境科学与工程前沿(英文)》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0898-6
关键词: Metal ions Biopolymer Activated sludge Three-dimensional excitation emission matrix (3D-EEM) Fluorescence regional integration (FRI) technique Quantification
A. DAVOUDINEJAD, P. PARENTI, M. ANNONI
《机械工程前沿(英文)》 2017年 第12卷 第2期 页码 203-214 doi: 10.1007/s11465-017-0421-6
Predictive models for machining operations have been significantly improved through numerous methods in recent decades. This study proposed a 3D finite element modeling (3D FEM) approach for the micro end-milling of Al6061-T6. Finite element (FE) simulations were performed under different cutting conditions to obtain realistic numerical predictions of chip flow, burr formation, and cutting forces. FE modeling displayed notable advantages, such as capability to easily handle any type of tool geometry and any side effect on chip formation, including thermal aspect and material property changes. The proposed 3D FE model considers the effects of mill helix angle and cutting edge radius on the chip. The prediction capability of the FE model was validated by comparing numerical model and experimental test results. Burr dimension trends were correlated with force profile shapes. However, the FE predictions overestimated the real force magnitude. This overestimation indicates that the model requires further development.
关键词: 3D finite element modeling micro end-milling cutting force chip formation burr formation
标题 作者 时间 类型 操作
Identification and ecotoxicity assessment of intermediates generated during the degradation of clofibric acid by advanced oxidation processes
Wenzhen LI, Yu DING, Qian SUI, Shuguang LU, Zhaofu QIU, Kuangfei LIN
期刊论文
spp., foodborne pathogens threatening neonates and infants
Qiming CHEN, Yang ZHU, Zhen QIN, Yongjun QIU, Liming ZHAO
期刊论文
Repeats in the transcribed regions: comprehensive characterization and comparison of
Manosh Kumar BISWAS, Christoph MAYER, Xiuxin DENG
期刊论文
Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells
LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan
期刊论文
Novel mutation c.1210-3C>G in with a poly-T tract of 5T affects mRNA splicing in a Chinese patient
期刊论文
Changes of phenotype and function of human CD4 CD25 T cells induced by transfection of Foxp3
WU Kui, BI Yutian, WANG Yaoli, WANG Changzheng
期刊论文
randomized trials of maintenance bacillus Calmette-Guerin instillation efficacy against recurrence of T1G3
PAN Jiangang, ZHOU Xing, CHEN Zhiguang, HAN Ruifa
期刊论文
Ductile extension of 3-D external circumferential cracks in pipe structures
Wuchao YANG, Xudong QIAN
期刊论文
An extended cell-based smoothed discrete shear gap method (XCS-FEM-DSG3) for free vibration analysis
Dang-TRUNG,T. NGUYEN-THOI
期刊论文
Interactions between metal ions and the biopolymer in activated sludge: quantification and effects of system pH value
Yun Zhou, Siqing Xia, Binh T. Nguyen, Min Long, Jiao Zhang, Zhiqiang Zhang
期刊论文